RESEARCH

We are excited about arthropod saliva!!!

Our main research is focused on the role of mosquito salivary proteins in the transmission of pathogens like Plasmodium, Zika virus and Dengue virus. We study the immune response of human cells (macrophages, mast cells and endothelial cells) against salivary proteins and how they shape clinical presentation of these diseases.

We also  study the antibody response (IgG, IgM and IgE) against arthropod saliva. In our most recent research, we have identified important salivary proteins from mosquitoes, ticks and triatomine bugs that can be used as markers for human-vector contact important to measure risk of disease.

Another important area of research in our lab is the study of the effect of human immune factors (complement proteins and antibodies) on the immune responses against pathogens in mosquito midgut. Our preliminary data suggest that the human complement proteins C5a and C3a induce important changes in gene expression of immune related genes in mosquitoes infected with Zika and Dengue viruses.

Publications

1: Vora A, Zhou W, Londono-Renteria B, Woodson M, Sherman MB, Colpitts TM, Neelakanta G, Sultana H. Arthropod EVs mediate dengue virus transmission through interaction with a tetraspanin domain containing glycoprotein Tsp29Fb. Proc Natl Acad Sci U S A. 2018 Jul 10;115(28):E6604-E6613. doi: 10.1073/pnas.1720125115. Epub 2018 Jun 26. PubMed PMID: 29946031; PubMed Central PMCID: PMC6048473.

 

2: Londono-Renteria BL, Shakeri H, Rozo-Lopez P, Conway MJ, Duggan N, Jaberi-Douraki M, Colpitts TM. Serosurvey of Human Antibodies Recognizing Aedes aegypti D7 Salivary Proteins in Colombia. Front Public Health. 2018 May 18;6:111. doi: 10.3389/fpubh.2018.00111. eCollection 2018. PubMed PMID: 29868532; PubMed Central PMCID: PMC5968123.

 

3: Londono-Renteria B, Troupin A, Cardenas JC, Hall A, Perez OG, Cardenas L, Hartstone-Rose A, Halstead SB, Colpitts TM. A relevant in vitro human model for the study of Zika virus antibody-dependent enhancement. J Gen Virol. 2017 Jul;98(7):1702-1712. doi: 10.1099/jgv.0.000833. Epub 2017 Jul 8. PubMed PMID: 28691657.

 

4: Hall A, Troupin A, Londono-Renteria B, Colpitts TM. Garlic Organosulfur Compounds Reduce Inflammation and Oxidative Stress during Dengue Virus Infection. Viruses. 2017 Jun 23;9(7). pii: E159. doi: 10.3390/v9070159. PubMed PMID: 28644404; PubMed Central PMCID: PMC5537651.

 

5: Londono-Renteria B, Marinez-Angarita JC, Troupin A, Colpitts TM. Role of Mast Cells in Dengue Virus Pathogenesis. DNA Cell Biol. 2017 Jun;36(6):423-427. doi: 10.1089/dna.2017.3765. Epub 2017 May 9. Review. PubMed PMID: 28486041.

 

7: Troupin A, Shirley D, Londono-Renteria B, Watson AM, McHale C, Hall A, Hartstone-Rose A, Klimstra WB, Gomez G, Colpitts TM. A Role for Human Skin Mast Cells in Dengue Virus Infection and Systemic Spread. J Immunol. 2016 Dec 1;197(11):4382-4391. Epub 2016 Oct 31. PubMed PMID: 27799312.

 

8: Londono-Renteria B, Cardenas JC, Troupin A, Colpitts TM. Natural Mosquito-Pathogen Hybrid IgG4 Antibodies in Vector-Borne Diseases: A Hypothesis. Front Immunol. 2016 Sep 29;7:380. eCollection 2016. PubMed PMID: 27746778; PubMed Central PMCID: PMC5040711.

 

9: Londono-Renteria B, Troupin A, Colpitts TM. Arbovirosis and potential transmission blocking vaccines. Parasit Vectors. 2016 Sep 23;9(1):516. Review. PubMed PMID: 27664127; PubMed Central PMCID: PMC5035468.

 

10: Conway MJ, Londono-Renteria B, Troupin A, Watson AM, Klimstra WB, Fikrig E, Colpitts TM. Aedes aegypti D7 Saliva Protein Inhibits Dengue Virus Infection. PLoS Negl Trop Dis. 2016 Sep 15;10(9):e0004941. doi: 10.1371/journal.pntd.0004941. eCollection 2016 Sep. PubMed PMID: 27632170; PubMed Central PMCID: PMC5025043.

 

11: Troupin A, Londono-Renteria B, Conway MJ, Cloherty E, Jameson S, Higgs S, Vanlandingham DL, Fikrig E, Colpitts TM. A novel mosquito ubiquitin targets viral envelope protein for degradation and reduces virion production during dengue virus infection. Biochim Biophys Acta. 2016 Sep;1860(9):1898-909. doi: 10.1016/j.bbagen.2016.05.033. Epub 2016 May 27. PubMed PMID: 27241849; PubMed Central PMCID: PMC4949077.

 

12: Londono-Renteria B, Colpitts TM. A Brief Review of West Nile Virus Biology. Methods Mol Biol. 2016;1435:1-13. doi: 10.1007/978-1-4939-3670-0_1. PubMed PMID: 27188545.

 

13: Londoño-Rentería B, Cárdenas JC, Giovanni JE, Cárdenas L, Villamizar P, Rolón J, Chisenhall DM, Christofferson RC, Carvajal DJ, Pérez OG, Wesson DM, Mores CN. Aedes aegypti anti-salivary gland antibody concentration and dengue virus exposure history in healthy individuals living in an endemic area in Colombia. Biomedica. 2015 Oct-Dec;35(4):572-81. doi: 10.7705/biomedica.v35i4.2530. PubMed PMID: 26844447.

 

14: Londono-Renteria B, Grippin C, Cardenas JC, Troupin A, Colpitts TM. Human C5a Protein Participates in the Mosquito Immune Response Against Dengue Virus. J Med Entomol. 2016 May;53(3):505-512. Epub 2016 Feb 3. PubMed PMID: 26843451; PubMed Central PMCID: PMC4892811.

 

15: Londono-Renteria B, Troupin A, Conway MJ, Vesely D, Ledizet M, Roundy CM, Cloherty E, Jameson S, Vanlandingham D, Higgs S, Fikrig E, Colpitts TM. Dengue Virus Infection of Aedes aegypti Requires a Putative Cysteine Rich Venom Protein. PLoS Pathog. 2015 Oct 22;11(10):e1005202. doi: 10.1371/journal.ppat.1005202. eCollection 2015 Oct. PubMed PMID: 26491875; PubMed Central PMCID: PMC4619585.

 

16: Londono-Renteria B, Drame PM, Weitzel T, Rosas R, Gripping C, Cardenas JC, Alvares M, Wesson DM, Poinsignon A, Remoue F, Colpitts TM. An. gambiae gSG6-P1 evaluation as a proxy for human-vector contact in the Americas: a pilot study. Parasit Vectors. 2015 Oct 13;8:533. doi: 10.1186/s13071-015-1160-3. PubMed PMID: 26464073; PubMed Central PMCID: PMC4605097.

 

17: Londono-Renteria B, Patel JC, Vaughn M, Funkhauser S, Ponnusamy L, Grippin C, Jameson SB, Apperson C, Mores CN, Wesson DM, Colpitts TM, Meshnick SR. Long-Lasting Permethrin-Impregnated Clothing Protects Against Mosquito Bites in Outdoor Workers. Am J Trop Med Hyg. 2015 Oct;93(4):869-74. doi: 10.4269/ajtmh.15-0130. Epub 2015 Jul 20. PubMed PMID: 26195460; PubMed Central PMCID: PMC4596613.

 

18: Chisenhall DM, Christofferson RC, McCracken MK, Johnson AM, Londono-Renteria B, Mores CN. Infection with dengue-2 virus alters proteins in naturally expectorated saliva of Aedes aegypti mosquitoes. Parasit Vectors. 2014 May 30;7:252. doi: 10.1186/1756-3305-7-252. PubMed PMID: 24886023; PubMed Central PMCID: PMC4057903.

 

19: Londono-Renteria B, Cardenas JC, Cardenas LD, Christofferson RC, Chisenhall DM, Wesson DM, McCracken MK, Carvajal D, Mores CN. Use of anti-Aedes aegypti salivary extract antibody concentration to correlate risk of vector exposure and dengue transmission risk in Colombia. PLoS One. 2013 Dec 2;8(12):e81211. doi: 10.1371/journal.pone.0081211. eCollection 2013. PubMed PMID: 24312537; PubMed Central PMCID: PMC3846924.

 

20: Londono-Renteria B, Eisele TP, Keating J, Bennett A, Krogstad DJ. Genetic diversity in the merozoite surface protein 1 and 2 genes of Plasmodium falciparum from the Artibonite Valley of Haiti. Acta Trop. 2012 Jan;121(1):6-12. doi: 10.1016/j.actatropica.2011.09.005. Epub 2011 Sep 29. PubMed PMID: 21982798.

21: Moudy RM, Michaels S, Jameson SB, Londono B, Lopez V, Caillouet KA, Hallmark CJ, Davis JK, Foppa IM, Dorn PL, Wesson DM. Factors associated with peridomestic Triatoma sanguisuga (Hemiptera: Reduviidae) presence in southeastern Louisiana. J Med Entomol. 2014 Sep;51(5):1043-50. PubMed PMID: 25276935.

 

22: Chisenhall DM, Londono BL, Christofferson RC, McCracken MK, Mores CN. Effect of dengue-2 virus infection on protein expression in the salivary glands of Aedes aegypti mosquitoes. Am J Trop Med Hyg. 2014 Mar;90(3):431-7. doi: 10.4269/ajtmh.13-0412. Epub 2014 Jan 20. PubMed PMID: 24445208; PubMed Central PMCID: PMC3945687.

 

23: Londono BL, Eisele TP, Keating J, Bennett A, Chattopadhyay C, Heyliger G, Mack B, Rawson I, Vely JF, Désinor O, Krogstad DJ. Chloroquine-resistant haplotype Plasmodium falciparum parasites, Haiti. Emerg Infect Dis. 2009 May;15(5):735-40. doi: 10.3201/eid1505.081063. PubMed PMID: 19402959; PubMed Central PMCID: PMC2686998.

 

24: Eisele TP, Keating J, Bennett A, Londono B, Johnson D, Lafontant C, Krogstad DJ. Prevalence of Plasmodium falciparum infection in rainy season, Artibonite Valley, Haiti, 2006. Emerg Infect Dis. 2007 Oct;13(10):1494-6. doi: 10.3201/eid1310.070567. PubMed PMID: 18257993; PubMed Central PMCID: PMC2851522.

 

25: Arango E, Londoño B, Segura C, Solarte Y, Herrera S, Saez J, Carmona-Fonseca J, Blair S. Prevention of sporogony of Plasmodium vivax in Anopheles albimanus by steroids of Solanum nudum Dunal (Solanaceae). Phytother Res. 2006 Jun;20(6):444-7. PubMed PMID: 16619357.

 

26: Londoño B, Arango E, Zapata C, Herrera S, Saez J, Blair S, Carmona-Fonseca J. Effect of Solanum nudum Dunal (Solanaceae) steroids on hepatic trophozoites of Plasmodium vivax. Phytother Res. 2006 Apr;20(4):267-73. Erratum in: Phytother Res. 2006 Jun;20(6):518. PubMed PMID: 16557608.

 

27: Londoño B, Carmona J, Blair S. [Comparison between OptiMAL and the thick smear tests for malaria diagnosis in an endemic area during a non-epidemic period]. Biomedica. 2002 Dec;22(4):466-75. Spanish. PubMed PMID: 12596444.